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To ensure optimal survival of any contaminants that may be in your samples, transportation on wet ice or on solid carbon dioxide is an integral part of the testing service.

For cell cultures, wet ice is the preferred method ("Collection and Transport of Specimens" W.A. Clyde Jr. and W.M. McCormack in "Methods in Mycoplasmology" Volume 1, C3, p. 103, Academic Press) which avoids the effects of variable ambient temperatures on the viability of mycoplasmas.

For Regulatory Testing, where mycoplasma testing is one of a range of tests applied to any given lot, a storage temperature of -65°C or below may be required in which case transport on solid carbon dioxide would be needed.

Containers will be delivered by courier with an Advice Note on the use of the contents and a specimen card. See packing instructions for Dry Ice and Wet Ice.

On the day of collection, cell cultures should ideally be in the exponential phase of growth and preferably in 25 cm² flasks. There is space in the container for several vessels of this size.  The collection day for Cell Screen and Cell Culture tests is Tuesday.

The organisation of delivery and collection unfortunately precludes the supply of wet ice or solid carbon dioxide. For temperature controlled transportation approximately two litres of crushed ice or five litres of solid carbon dioxide pellets will be required on the day of collection.

Containers with samples will be collected and delivered to our laboratories the following morning for test initiation.

Please note that only pathogens belonging to Groups 1 and 2* can be carried. Where testing of cultures exposed to Groups 3 or 4 is required, please contact  Mycoplasma Experience.

* Advisory Committee on Dangerous Pathogens "Categorisation of pathogens according to hazard and categories of containment" 1984. HMSO.

Click here to link to instructions for packaging samples on wet ice or dry ice.