Mycoplasma Experience is now offering two real time (RT)-
DNA is extracted from the test article using the Roche “MagNA Pure Compact” robot.
Real time amplification of samples is carried out in optical reaction plates with TaqMan® probes using the Venor®Gem qEP mycoplasma detection kit for qPCR (Minerva biolabs) OR the MycoTOOL Real-
Rapid Microbiological Mycoplasma Testing -
Protocol 1 -
Suitable for Cell Therapy or Advanced Therapy Medicinal Products (ATMPs)
This test may be included for an early result whilst Cultural Mycoplasma Tests are ongoing.
Both the Venor®Gem qEP test kit and the MycoTOOL kit used comply fully with the requirements of European Pharmacopoeia 2.6.7 and we have further validated the sensitivity of our protocol to 10cfu/mL. See here for data. A validation will be required for all new sample types to be tested. Please contact us for costings.
In addition to the standard positive controls, the test will include three positive low number spike controls in the test matrix to confirm absence of matrix inhibition.
Price: One test -
Each additional sample processed concurrently-
The standard protocol for rapid mycoplasma detection by PCR in cell cultures or other non-
Price: 8 or more samples submitted together -
Fewer than 8 samples -
Transport of Samples To Our Laboratory
Samples can be sent independently, or we can arrange to send a cool box out to you and collect it by courier for delivery to us, or you can use your own packaging and we can just arrange courier collection.
Full Sample transport service -
Collection of your sample container only -