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Mycoplasma Experience is can offer a Real Time PCR protocol aimed at cell cultures and Cell Therapy or Advanced Therapy Medicinal Products (ATMPs) for rapid mycoplasma testing. We hold a MHRA license, allowing us to carry out regulatory tests on therapeutic clinical products. See here for a copy of our license.



Rapid Microbiological Mycoplasma Testing - PCR

Protocol 1 - Testing according to Eur. Ph. 2.6.7

Suitable for Cell Therapy or Advanced Therapy Medicinal Products (ATMPs)

This test may be used as a standalone test or included for an early result whilst Cultural Mycoplasma Tests are ongoing.

The test

DNA is extracted from the test article using the Qiagen EZ2 Connect Instrument.

Real time amplification of samples is carried out in optical reaction plates with the MycoTOOL Real-Time PCR Kit (Roche) in a Roche LightCycler® 480.

Please contact us for validation information and costing.


Protocol 2- For screening of routine cell cultures and research samples

The standard protocol for rapid mycoplasma detection by Real Time PCR in cell cultures or other non-regulatory samples. A test of inhibition using live Mycoplasma spikes can be done before routine testing is initiated.

Price: 1 sample -                      £150.00 per sample

2 samples -                    £105.00 per sample

3 samples -                    £90.00 per sample

4-9 samples-                  £80.00 per sample

10+ samples-                 £60.00 per sample


Transport of Samples To Our Laboratory

Samples can be sent independently, or we can arrange to send a cool box out to you and collect it by courier for delivery to us, or you can use your own packaging and we can just arrange courier collection.

Full Sample transport service                      -                          £50.00

Collection of your sample container only   -                          £25.00


Mycoplasma Experience Ltd.                               Phone: 01883 740185                    Email: mexp@mycoplasma-exp.com