CELL CULTURE TESTING
Two cultural tests are offered, a cell-
We inoculate onto properly validated mycoplasma media with tests conducted by expert and experienced personnel -
Your samples are tested on our unique media formulations developed to allow growth of cell-
Because we don’t have to rely on less sensitive indirect tests to detect these organisms there are no false positives and a reduced likelihood of false negatives.
We give prompt notification of positives so you can take remedial action (negative tests must complete the full 21-
We can advise on sample point selection, extent and frequency of testing and, where necessary, communicate with Licensing Authorities regarding mycoplasma testing of your products.
For cell culture contamination and eradication please see our consultancy pages.
Sample collection and temperature controlled transport is an integral part of this service (see sample transportation for wet ice and solid carbon dioxide transportation).
MYCOPLASMAS: THE CELL BIOLOGIST’S HIDDEN ENEMY
Cell cultures provide an ideal cultural environment for contaminating mycoplasmas which, once an infection is established, can spread rapidly to other cell lines in the laboratory. Mycoplasmas have been recognised as common contaminants of mammalian cell cultures for many years and they should not be overlooked as potential contaminants of insect or plant cell lines.
Whilst mycoplasmas, unlike bacterial or fungal contaminants, may not reveal their presence they can have a profound effect on cell cultures. To help you appreciate how mycoplasma infection could affect your work, Mycoplasma Experience has prepared a microfilm sheet of over 200 selected references on cell culture infection with mycoplasmas. (This is available on request and may be read under a microscope at low magnification if a viewer is unavailable).
Regular testing for mycoplasmas is the only way to be sure of working with mycoplasma-
TESTING FOR MYCOPLASMAS
A combination of direct culture on mycoplasma media and an indirect, or non-
The existence of "non-
M. orale (M. Merkenschlager et al. Immunology 1988, 63, 125-
Extensive media development by Mycoplasma Experience scientists has resulted in media which support the growth of M. hyorhinis cultivar a as well as many mycoplasma species recognised as difficult to culture including fastidious strains of M. orale and M. fermentans. (The strain of M. orale encountered by Merkenschlager is used routinely for quality control of media). Use of these media for cell culture testing eliminates the need for additional testing methods and permits detection of low level infection of both fastidious and less culturally demanding strains independently of their capacity to cytadsorb.